My first research experience was with the Hernández Sánchez group at the University of Pittsburgh. There, I worked on the synthesis of radially oriented [n]cyclo-meta-phenylenes, and gained proficiency research skills such as chromatography and NMR analyses.
Following graduation, I joined the Buehler Lab at the Center for Blood Oxygen Transport and Hemostasis (CBOTH). During my time there I worked on an array of studies, from sickle cell and COVID-based coagulopathies to drug induced hemolysis. I developed laboratory methods, such as an assay for the generation of methemoglobin in response to oxidative insult and worked to refine existing methods on HPLC separation of modified hemoglobins. I also worked on the logistics of study design and implementation, including the initiation of a clinical study of cardiopulmonary bypass related hemolysis. Much of my work concerned the development of polymerized hemoglobin-based oxygen carriers and their evaluation in vivo.
During my time at CBOTH, I realized that while I had an interest in researching disease outcomes and hemorrhage therapeutics, it lay more with the public health impact of these issues, and that I had a growing interest in developing statistical methodology. For those reasons, I left CBOTH and decided to pursue an M.S. at Columbia Mailman. My full resume can be found here.
I am currently working in the Center for Statistical Genetics in the Gertrude H. Sergievsky Center at Columbia. My current research focuses on methods for integrating genomic and proteomic data.
My work on the above topics has been published in the articles below and presented at conferences in the subsequent abstracts section.
Dziewulska-Cronk KH, Reisz JA, Hay AM, Nemkov T, Cendali FI, Issaian A, Lamb DR, Palha MS, Legenzov EA, Kao JPY, Walker LA, Tekwani BL, Buehler PW, D’Alessandro A, Zimring JC. Primaquine-5,6-orthoquinone is directly hemolytic to older G6PD deficient RBCs in a humanized mouse model. J Pharmacol Exp Ther. 2024 Aug 2:JPET-AR-2024-002218. doi: 10.1124/jpet.124.002218.
Cuddington C, Greenfield A, Lee YG, Kim JL, Lamb D, Buehler PW, Black SM, Palmer AF, Whitson BA. Polymerized Human Hemoglobin-Based Oxygen Carrier Preserves Lung Allograft Function During Normothermic Ex Vivo Lung Perfusion. ASAIO J. 2024 Jan 25. doi: 10.1097/MAT.0000000000002118.
Lamb DR, Greenfield A, Thangaraju K, Setua S, Eiker G, Wang Q, Vahedi A, Khan MA, Yahya A, Cabrales P, Palmer AF, Buehler PW. The Molecular Size of Bioengineered Oxygen Carriers Determines Tissue Oxygenation in a Hypercholesterolemia Guinea Pig Model of Hemorrhagic Shock and Resuscitation. Mol Pharm. 2023 Oct 16. doi: 10.1021/acs.molpharmaceut.3c00611.
Palha MS, Legenzov EA, Lamb DR, Zimring JC, Buehler PW, Kao JPY. Superoxide measurement as a novel probe of red blood cell storage quality. Blood Transfus. 2023 Sep;21(5):422-427. doi: 10.2450/2022.0246-22.
Greenfield A, Lamb DR, Gu X, Thangaraju K, Setua S, Yahya A, Vahedi A, Khan MA, Wang Q, Buehler PW, Palmer AF. Biophysical Analysis and Preclinical Pharmacokinetics-Pharmacodynamics of Tangential Flow Filtration Fractionated Polymerized Human Hemoglobin as a Red Blood Cell Substitute. Biomacromolecules. 2023 Apr 10;24(4):1855-1870. doi: 10.1021/acs.biomac.3c00051.
Thangaraju K, Setua S, Lisk C, Swindle D, Stephenson D, Dzieciatkowska M, Lamb DR, Moitra P, Pak D, Hassell K, George G, Nuss R, Davizon-Castillo P, Stenmark KR, D’Alessandro A, Irwin DC, Buehler PW. Extracellular Vesicle Size Reveals Cargo Specific to Coagulation and Inflammation in Pediatric and Adult Sickle Cell Disease. Clin Appl Thromb Hemost. 2023 Jan-Dec;29:10760296231186144. doi: 10.1177/10760296231186144.
Setua S, Thangaraju K, Dzieciatkowska M, Wilkerson RB, Nemkov T, Lamb DR, Tagaya Y, Boyer T, Rowden T, Doctor A, D’Alessandro A, Buehler PW. Coagulation potential and the integrated omics of extracellular vesicles from COVID-19 positive patient plasma. Sci Rep. 2022 Dec 23;12(1):22191. doi: 10.1038/s41598-022-26473-8.